Antibody-mediated lysis of PNH clone. The graphs represent MIRL-positive cells on the y-axis in a PE channel. The x-axis represents a blank fluorescein isothiocyanate channel. A group O RBC sample with a clone of patient PNH RBCs was used for the study. (A) Negative control: RBCs sensitized with pooled AB plasma. (B) An 86% reduction in the size of PNH clone was observed upon sensitization of RBCs with anti-I because of the lysis of PNH clone.