Flow cytometric evaluation of MIRL. The graphs represent fluorescence counts on the y-axis vs channel PE (MIRL) on the x-axis. (A) Untreated group A1 RBCs expressing MIRL, (B) AET-treated group A1 RBCs lost MIRL and have an overlapping profile with the isotype control and unstained RBCs. Green peak, isotope control; pink peak, unstained control; purple peak, MIRL-positive cells.