Figure 1.
![Figure 1. Generation of FVIII-specific CD4 T cells from healthy individuals. CD4 T-cell lines were generated as described in Material and Methods. (A) FVIII-specific T-cell lines generated from donor #329. (B) Cross-reactivity analysis between KLH- and FVIII-specific T-cell lines. (C) Frequencies of CD4 T cells specific for FVIII and KLH estimated for 16 healthy blood donors. The frequency of CD4 T-cell precursors was calculated using Poisson distribution according to the following formula: Frequency = −Ln([number of negative wells/total number of wells tested])/(number of CD4 T cells per well). Statistical significance was assessed using the Wilcoxon match-pairs signed rank test. ****P < .0001. Ctrl, control.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/1/21/10.1182_bloodadvances.2017008706/3/advances008706f1.jpeg?Expires=1724943715&Signature=EMoirHbTdS0uvk1CrNC3A3y0qsF9TcFhojXC256idmWKaXtIMTTtEEIjFSG9RUJ~iq12fU4fwtFFp8dtKuTyH8pPHGo~UWvjXf6MRG2xBuMs31MuNepdVfU7Po2hSRS2Xc1DsdnHRaY8Yq9W4U2ImLoUyrMoLOYixR238fRz6usqjZZbs21MBISnPz~DTZX-M7vlx99fIClA1Q-4McRVnQrigTWLgwTzT45zXXEtcvLwWeGyZ5NNxnPpJwB3MVzVRWMT3AzwCLj5j7hEvfgfwNGFdIOgGlNOYbHFP28mWA83K7-OgN1DnAjo8shy9KWJ5YWpmdi23Owif2YhGu-hKA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Generation of FVIII-specific CD4 T cells from healthy individuals. CD4 T-cell lines were generated as described in Material and Methods. (A) FVIII-specific T-cell lines generated from donor #329. (B) Cross-reactivity analysis between KLH- and FVIII-specific T-cell lines. (C) Frequencies of CD4 T cells specific for FVIII and KLH estimated for 16 healthy blood donors. The frequency of CD4 T-cell precursors was calculated using Poisson distribution according to the following formula: Frequency = −Ln([number of negative wells/total number of wells tested])/(number of CD4 T cells per well). Statistical significance was assessed using the Wilcoxon match-pairs signed rank test. ****P < .0001. Ctrl, control.
