Lymphocytes isolated from lymph nodes express the Vγ8Vδ1-TCR. (A) Flow cytometry of cell suspension prepared from the left inguinal lymph node isolated from Pt. 1. Cell numbers are expressed as a percentage of total lymphocytes. (B) Flow cytometry on the same cell suspension but stained for CD4 and CD8 and gated on CD3+/CD45+ cells. (C) Flow cytometry analysis of γδTCR and αβTCR expression by CD3+/CD45+ cells present in the same inguinal lymph node. (D-E) Sequencing of recombined TCR genes in cells isolated from the lymph nodes from Pt. 1 (empty bars) and Pt. 2 (filled bars), showing Vγ and Vδ TCR chain usage. (F) The Vγ8 chain CDR3 length distribution in Pt. 1 (empty bars) and Pt. 2 (filled bars). (G-J) A network analysis for visualizing the sequences’ similarity, frequency, and level of homology. Each node in the network represents a sequence, and each color represents a different Vδ (G-H) or Vγ (I-J) chain. The size of each node is proportional to the frequency of the amino acid sequence. The level of homology between sequences is shown by the connecting lines.
