Reduced inflammatory cytokine expression in CIA-challenged uPA- and uPAR-deficient mice. (A-C) Quantitative RT-PCR analysis of messenger RNA levels for the inflammatory cytokines TNF-α (A), IL-1β (B), and IL-6 (C) in the hindpaws obtained from unchallenged WT and Plau^−/− mice (n = 5 and 4, respectively) and day 42 of CIA WT and Plau^−/− mice (n = 8 per genotype). (D-F) Quantitative RT-PCR analysis of mRNA levels for the inflammatory cytokines TNF-α (D), IL-1β (E), and IL-6 (F) in the hindpaws obtained from unchallenged wild-type and Plaur^−/− mice (n = 6 per genotype) and day 42 of CIA WT and Plaur^−/− mice (n = 6 per genotype). Data are expressed as average fold change over unchallenged WT group with error bars denoting standard error of the mean. Data were analyzed by 2-way analysis of variance followed by Student-Newman-Keuls post hoc test. #P < .05 for comparing unchallenged and CIA-challenged of the same genotype, *P < .05 for comparing WT to Plau^−/− or Plaur^−/− at day 42 of CIA, and **P < .01 for comparing WT to Plau^−/− or Plaur^−/− at day 42 of CIA.