Figure 1.
![Figure 1. Major O-glycan structures in human serum and endothelial cells. The majority of O-glycans in human serum4 and HUVECs5 are formed from sialylated Core 1 (Galβ1-3GalNAcαSer/Thr) and Core 2 (GlcNAcβ1-6[Galβ1-3] GalNAcαSer/Thr) structures. Mature glycans are capped with variable numbers of negatively charged sialic acid (Neu5Ac), which can be removed enzymatically by neuraminidases. O-glycans can also be found in fucosylated and sulfated forms. An example of a complex Core 2 O-glycan carrying a 6-O-sulfated sialyl-Lewis x terminal structure is shown at right. The lectin enrichment strategy employed in this study uses VVA and PNA to predominantly target the biosynthetic intermediate, Tn, and Core 1 O-glycans.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/1/7/10.1182_bloodadvances.2016002121/3/advances002121f1.jpeg?Expires=1730501349&Signature=kPi~rtmT~ATDc9ddIqLe2GrxJ3AlhlV-Gp78uFK2niL2sKOdMa4Q9gpCUm0w4-NOcaFdOnTN~5hdw7kjWj8WWtwaKyAcjNbYJ20UcjEkAWTzgd4P20ND9SeJd9F4-KgHGZczNHC9ceDhI0qCnkx8F-xTtRF7~24LQL42~RZ11oCkvRtQ-Al7q4bYqOStuqdXaij8cFU4Do4kR-7xYFHDRj4TnfLkOjedE6VqdnDui6aBsLk7KE-6z8A8Je3rddwFjFfKL56HpbKJcm1TE1k9S81eRMCS3M5F6WR6ZeJlnlmEeX3kUeVXgJB92iUEMWrCgiPRod7vxbuaFZ8yc2gPTw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Major O-glycan structures in human serum and endothelial cells. The majority of O-glycans in human serum4 and HUVECs5 are formed from sialylated Core 1 (Galβ1-3GalNAcαSer/Thr) and Core 2 (GlcNAcβ1-6[Galβ1-3] GalNAcαSer/Thr) structures. Mature glycans are capped with variable numbers of negatively charged sialic acid (Neu5Ac), which can be removed enzymatically by neuraminidases. O-glycans can also be found in fucosylated and sulfated forms. An example of a complex Core 2 O-glycan carrying a 6-O-sulfated sialyl-Lewis x terminal structure is shown at right. The lectin enrichment strategy employed in this study uses VVA and PNA to predominantly target the biosynthetic intermediate, Tn, and Core 1 O-glycans.
