Three-dimensional imaging of BM. (A) Representative stages of murine femoral bones during processing for 3D imaging as described in Nombela-Arrieta et al.13 From left to right: unsectioned, sliced unprocessed, and optically cleared BM slices. The last 2 images to the right correspond to an example of 3D reconstruction of the entire BM microvascular system of the femur (arteries and sinusoids) using multidimensional confocal imaging of a cleared BM slice. A detailed view of the central sinus running along the longitudinal axis of the marrow is provided. (B) High-resolution 3D imaging of a reduced field of view showing sinusoidal vessels (red) and the network of perivascular bodies of CAR cells (green), forming a dense matrix through the emission of abundant cytoplasmic projections.
