American Society of Hematology

Figure 3.

$Figure 3. Functional LSC activity in myeloid BP-CML. (A) Purities of immunophenotypic populations (expressed as percentage), after FACS sorting, used in primary xenotransplantation from 5 patients (COL091, COL091R, CML371, CML002, and HER002). (B) Number of mice with human cell engraftment above engraftment threshold (defined as 0.1% human CD45+CD33+CD19− cells) out of the total number of mice injected. Myeloid engraftment or absence of engraftment is indicated. ND, nondetected. (C) Primary engraftment (4 patients: COL091, COL091R, CML371, and CML002, x-axis) in 1 to 6 mice from the indicated population. Black and red dots show the engraftment level in individual mice. y-axis: mean percentage human (h)CD45+CD33+CD19− cell engraftment/total live MNC. x-axis: injected cell fraction. Red line: engraftment threshold. (D) Number of human cells (y-axis) injected in primary mice from indicated population from 4 patients: COL091, CML371, COL091R, and CML002 (x-axis) in 1 to 6 mice. Black dots: engrafted mice. Red dots: mice that failed to engraft above the engraftment level of 0.1%.$

Functional LSC activity in myeloid BP-CML. (A) Purities of immunophenotypic populations (expressed as percentage), after FACS sorting, used in primary xenotransplantation from 5 patients (COL091, COL091R, CML371, CML002, and HER002). (B) Number of mice with human cell engraftment above engraftment threshold (defined as 0.1% human CD45⁺CD33⁺CD19⁻ cells) out of the total number of mice injected. Myeloid engraftment or absence of engraftment is indicated. ND, nondetected. (C) Primary engraftment (4 patients: COL091, COL091R, CML371, and CML002, x-axis) in 1 to 6 mice from the indicated population. Black and red dots show the engraftment level in individual mice. y-axis: mean percentage human (h)CD45⁺CD33⁺CD19⁻ cell engraftment/total live MNC. x-axis: injected cell fraction. Red line: engraftment threshold. (D) Number of human cells (y-axis) injected in primary mice from indicated population from 4 patients: COL091, CML371, COL091R, and CML002 (x-axis) in 1 to 6 mice. Black dots: engrafted mice. Red dots: mice that failed to engraft above the engraftment level of 0.1%.

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