Target cell–induced function is impaired at D+28 after dUCBT for NK cell groups. PBMCs from healthy donor controls and patients at D+28 after dUCBT were cocultured with K562 target cells for 4 hours or IL-12/IL-18 overnight. Expression of CD107a, IFN-γ, and TNF-α was measured on CD56⁺Lin⁻ gated NK cells. (A) Expression of IFN-γ after K562 coculture (left) or IL-12/IL-18 stimulation (right). (B) Expression of TNF-α after K562 coculture. (C) Expression of CD107a after K562 coculture. Data represented as a data point for each patient with the mean of the population as the solid middle line with standard error above and below. Healthy donor controls are represented by the mean (dashed line) and standard error (gray box). For samples shown in panels D-I, NK cells were gated on CD56^bright and CD56^dim populations based on MFI. Percentage expression of IFN-γ (D,G), TNF-α (E,H), and CD107a (F,I) is shown.