Kinetics of KRH3955-induced mobilization of leukocytes and HPCs and studies on the bone marrow:blood CXCL12 chemokine gradient. (A-D) Mice were administered KRH3955 (30 mg/kg mouse by oral gavage) or vehicle (dash), and at varying time points (2, 6, and 24 hours), blood was collected for analysis of circulating TNCs (A) and CFU-HPCs (B) (n = 7-10 mice per group). Femoral bone marrow and peripheral blood were collected for quantification of CXCL12 in BM supernatant (C) and PB plasma (D), respectively (n = 7-17 mice per group). CXCL12 levels are shown as picograms per milliliter. Data from at least 2 independent experiments are represented as mean ± SEM. ***P < .001 (one-way ANOVA with Dunnett test). (E-F) Mice were administered KRH3955 or vehicle (dash) in the presence or absence of C4P, and 2 hours later, blood was collected for analysis of circulating TNCs (E) and CFU-HPCs (F) (n = 4-8 mice per group). TNCs and CFU-HPCs are shown as cells and colonies per milliliter of blood, respectively. Data from 2 independent experiments are represented as mean ± SEM. *P < .05; **P < .01; ***P < .001 (one-way ANOVA with Bonferroni test).