Histological analysis of mouse major organ tissues. Adamts13^−/− mice (CAST/Ei) were treated with PBS or anfibatide (60 ng/g body weight) as indicated twice daily for 3 days after a single administration of Stx-2 (250 pg/g body weight). Major organ tissues from brain (A, G), heart (B, H), lung (C, I), liver (D, J), and kidney (E, K), as well as spleen (F, L) were collected from 3 mice in each group (either naturally died or sacrificed 3 days of the initial Stx-2 challenge) and processed for hematoxylin and eosin staining. Panels A-F are the control groups; panels G-L are the anfibatide-treated group here. Arrows indicate the presence of microvascular thrombosis. The images are representatives of all major organ tissues from 3 mice in each group. Original magnification ×200 for all panels.