Figure 1.
Effect of anfibatide on botrocetin-induced agglutination of murineplatelets. Washed murine platelets (2 × 108/mL) were incubated with various concentrations (0, 3, 6, 9, and 12 μg/mL or 0 and 9 μg/mL) of purified anfibatide in the presence of mVWF (10 μg/mL). (A-B) The botrocetin (1 μg/mL) and PAR-4 agonist (AYPGKF) (500 μM) induced platelet agglutination, respectively. The change in light transmission (%) was recorded continuously for 3 minutes and plotted against the time. Each experiment was repeated 3 times. Only the representative images and tracing are shown.
