Twenty-seven–gene panel DNA sequence variants in Mayo Clinic patients with PV (n = 133) and ET (n = 183). (A,C) Individual variant/mutational cosegregation plot for both PV and ET. Each column represents 1 of the sequenced subjects. Variant/mutations are depicted by representative colored bars. Red, Variants previously associated with a hematologic malignancy, identified as being somatic, and present with ≤1% minor allele frequency (MAF). Pink, Variants previously associated with a hematologic malignancy and present with ≤1% MAF. Blue, Variants not previously associated with a hematologic malignancy and present with ≤1% MAF. (B,D) Variant/mutation totals in PV and ET ranked by gene and corresponding overall frequency percentage.