American Society of Hematology

Figure 3.

$Figure 3. Erythrophagocytosis by neutrophils is strongly dependent on the degree of opsonization of the RBCs. (A) Anti-GPA shows a much higher mean fluorescence intensity (MFI) compared with anti-D after secondary antibody staining (n = 3). (B) Phagocytosis by neutrophils depends on the degree of opsonization; extent of phagocytosis explained as MFI of anti-GPA-opsonized RBCs (left) or the percentage of phagocytic neutrophils (right) (n = 10). Error bars denote the standard error of the mean. (C-D) Cytospins of the phagocytic fraction using (C) anti-RhD-opsonized RBCs (original magnification ×50; May-Grünwald Giemsa stain) and (D) anti-GPA-opsonized RBCs (original magnification ×50; May-Grünwald Giemsa stain). These images were blindly chosen and are representative for 5 different experiments.$

Erythrophagocytosis by neutrophils is strongly dependent on the degree of opsonization of the RBCs. (A) Anti-GPA shows a much higher mean fluorescence intensity (MFI) compared with anti-D after secondary antibody staining (n = 3). (B) Phagocytosis by neutrophils depends on the degree of opsonization; extent of phagocytosis explained as MFI of anti-GPA-opsonized RBCs (left) or the percentage of phagocytic neutrophils (right) (n = 10). Error bars denote the standard error of the mean. (C-D) Cytospins of the phagocytic fraction using (C) anti-RhD-opsonized RBCs (original magnification ×50; May-Grünwald Giemsa stain) and (D) anti-GPA-opsonized RBCs (original magnification ×50; May-Grünwald Giemsa stain). These images were blindly chosen and are representative for 5 different experiments.

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