Enrichment of DNA cis-elements in enhancers that display an open chromatin configuration in hematopoietic cells. (A) The overlap of enhancers displaying an open chromatin state in LT-HSCs, ST-HSCs, and MPPs. (B) The overlap of enriched transcription factor–binding motifs found in both promoters and enhancers that display an open chromatin state in our ATAC-seq analysis. (C) PCA for the enriched DNA motifs in open enhancers within the 3 cell types. The first 2 components account for 99.21% and 0.79% of the total variance in the dataset, respectively. Dots represent the PCA loadings for the 153 transcription factors. The different colored dots represent 3 different cohorts. (D-H) Metaplot of ATAC-seq signal around the flanking 1 kb of PU.1, SPIB, ELF5, CCCTC-binding factor (CTCF), and BORIS binding motifs. (I) Venn diagram showing the overlap of poised enhancers in LT-HSCs and ST-HSCs and active enhancers in MPPs.