IL-2 amplifies the response of ILC2s to ECs. ILC2s were cultured for 5 days in the presence of EC ± IL-2. (A) Percentage of ILC2s expressing intracellular IL-13 following activation with EC ± IL-2 for 5 days and 4-hour incubation with brefeldin A prior to cell staining. Data depict 3 independent experiments (n = 2 donors/experiment). (B) GM-CSF production was also analyzed in the cells supernatants following a 5-day in vitro culture. Data depict 2 independent experiments (n = 2 donors). Data are shown as mean ± SEM from 2 independent experiments, with n = 2 donors each. Significance was calculated using 1-way ANOVA followed by correction for multiple comparisons, where *P < .05, **P < .01, ***P < .001, and ****P < .0001. Statistics above bars represent comparison with media baseline; comparison between samples is represented by the connecting line. (C) Representative contour plots of granzyme B expression by ILC2s cultured with EC ± IL-2 for 5 days and incubation with brefeldin A for 4 hours prior to intracellular cell staining. ns, not significant.