ILC2 cytokine secretion pattern following stimulation with different combinations of ECs in IMDM or SFEM II culture media. ILC2s were stimulated with the triple EC combination or with all 3 ECs plus IL-2, and effector cytokine production was quantified in the supernatants after 5 days in culture with either IMDM or SFEM II. (A-E) Multiplexed ELISA was performed to measure IL-13 (A), IL-5 (B), IL-4 (C), IL-8 (D), and IFN-γ (E). Data are shown as mean ± SEM from 2 independent experiments, with n = 2 or 3 donors each. Significance was calculated using 1-way ANOVA followed by correction for multiple comparisons, where *P < .05, **P < .01, ***P < .001, and ****P < .0001. Statistics above bars represent comparison with media baseline; comparison between samples is represented by the connecting line. (F) Representative contour plots showing intracellular expression of IL-13, IL-5, and IFN-γ in ILC2s cultured in either IMDM or SFEM II following 4-hour incubation with brefeldin A. Data are representative of 2 independent experiments (n = 2 donors). ns, not significant.