viSNE map of CD45+ cells from a patient with DLBCL arising from a CLL background. Each unit on the dot plot represents a cell. Their coordinates on the map are functions of their phenotypic distance. Major subsets were identified using unbiased clustering and annotated (A). Events were color coded according to the expression of representative markers (B-G). A heat map (H) displays median intensity of expression of a comprehensive set of markers included in the panel for each immune subset.