LAPTM5 gene suppressed K562 cells' autophagy activity. K562 cells transfected with LAPTM5(L) and empty vector (P) were cultured in HBSS conditional media for 6 hrs. On the time point of 0hrs, 3hrs and 6hrs, transfected cells were collected and run Westernblot with the antibody of LC3-II and LC3-I. The ratio of LC3-II/LC3I which represented the extent of autophagy activity decreased in K562/LAPTM5 (L).