Regulation of IRF8 Expression. A. Structure of IRF8 transcripts. Black bent arrows - start codons; splice donor/acceptor cites connected by dotted lines; pIRE methylation - CH₃; primers - black half-arrows; probe - green solid line. B. Figure shows methylation (top) and expression (bottom) of IRF8. Cells from normal donors and lymphocytes (CD3) from AML BM show no evidence of methylation of pIRE and higher expression. AML blasts (CD34) are methylated and show a significant decrease of IRF8 expression. C. Figure shows restoration of IRF8-WT and decrease of IRF8-SVs expression in U937 exposed to 5-Aza.