Walters Figure 3.
Gene therapy with hematopoietic stem cells (HSCs) and post-transduction selection.42
Limiting numbers of MGMT-expressing gene-corrected cells are expanded by initially killing most of the of untransduced HSCs with BCNU and O6-benzylguanine (O6BG) therapy. Self-renewal and expansion of MGMT gene-corrected HSCs repopulate all hematopoietic lineages.
Reprinted with permission from
Bank A. Hematopoietic stem cell gene therapy: selecting only the best. J Clin Invest. 2003;112(10):1478–1480.
