Figure 4.
Figure 4. Lentiviral vector design with the cHS4 insulator.33 The SIN lentiviral backbone is flanked by a 1.2-kb cHS4 insulator (cHS4-I) element to replace the 398-bp U3 promoter and/or enhancer deletion. Upon proviral integration into host genome, the U3 region containing the cHS4 is copied over to the 5′LTR. The ‘insulated’ replacement gene contains the β-globin gene and β-globin LCR elements. / Reprinted with permission from Puthenveetil G, Scholes J, Carbonell D, et al. Successful correction of the human beta-thalassemia major phenotype using a lentiviral vector. Blood. 2004;104:3445–3453. ©American Society of Hematology.

Lentiviral vector design with the cHS4 insulator.33 The SIN lentiviral backbone is flanked by a 1.2-kb cHS4 insulator (cHS4-I) element to replace the 398-bp U3 promoter and/or enhancer deletion. Upon proviral integration into host genome, the U3 region containing the cHS4 is copied over to the 5′LTR. The ‘insulated’ replacement gene contains the β-globin gene and β-globin LCR elements.

Reprinted with permission from

Puthenveetil G, Scholes J, Carbonell D, et al. Successful correction of the human beta-thalassemia major phenotype using a lentiviral vector. Blood. 2004;104:3445–3453.
©American Society of Hematology.

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