Figure 7
Figure 7. Loss of mDia1 led to a downregulation of membrane-associated genes and a specific upregulation of CD14 mRNA in granulocytes. (A-B) Heat map comparing RNA sequencing gene expression profiles of granulocytes (Gr1+, Mac1+) of bone marrow WT vs Het (A, left panel), and WT vs KO mDia1 mice (B, left panel). Arrows indicate CD14 overexpression in mDia1 Het and KO Gr1/Mac1+ cells. Gene set enrichment analysis shows decreased expression of a plasma membrane signature (right panels). N = 4 in each genotype group. Mice used were 6-8 weeks old. (C) Real-time PCR analysis of CD14 mRNA levels from the indicated cells. (D) The cells were cultured as in Figure 3G. CD14 mRNA levels of the indicated cells were analyzed using real-time PCR. (E) Real-time PCR analysis of CD14 and DIAPH1 levels in indicated blood lineages from WT mouse (6-8 weeks old).

Loss of mDia1 led to a downregulation of membrane-associated genes and a specific upregulation of CD14 mRNA in granulocytes. (A-B) Heat map comparing RNA sequencing gene expression profiles of granulocytes (Gr1+, Mac1+) of bone marrow WT vs Het (A, left panel), and WT vs KO mDia1 mice (B, left panel). Arrows indicate CD14 overexpression in mDia1 Het and KO Gr1/Mac1+ cells. Gene set enrichment analysis shows decreased expression of a plasma membrane signature (right panels). N = 4 in each genotype group. Mice used were 6-8 weeks old. (C) Real-time PCR analysis of CD14 mRNA levels from the indicated cells. (D) The cells were cultured as in Figure 3G. CD14 mRNA levels of the indicated cells were analyzed using real-time PCR. (E) Real-time PCR analysis of CD14 and DIAPH1 levels in indicated blood lineages from WT mouse (6-8 weeks old).

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