Mice with mDia1 deficiency developed age-related MDSs. (A) Transplantation of total bone marrow cells from indicated mice (CD45.2⁺) to lethally irradiated mice (CD45.1⁺). Flow cytometric analysis of CD45.2⁺ cells in the peripheral blood was performed 1 month after transplantation. WT: N = 6, Het: N = 5, KO: N = 3. (B) Same as in panel A except that Gr1/Mac1 double-positive cells were analyzed. N = 7 in each group. (C) Transplantation of LSK cells (CD45.2⁺) from indicated mice to lethally irradiated recipient mice (CD45.1⁺). The percentage of peripheral blood CD45.2⁺ cells was determined 1 month after transplantation. N = 3 in each group. (D) Same as in panel C except that Gr1⁺ cells were analyzed by flow cytometry. (E) Colony-forming unit (CFU) assay of various hematopoietic lineages derived from LSK cells of indicated mice (6-8 weeks of age). BFU-E, burst-forming unit, erythrocytes; G, granulocytes; GEMM, granulocytes, erythrocytes, monocytes/macrophages; GM, granulocytes, monocytes; M, monocytes. Data were representative of at least 3 independent experiments. (F) White blood cell and neutrophil counts of mice 1 month after transplantation with mDia1 wild-type, heterozygous, and knockout total bone marrow cells. (G) Wright-Giemsa stains of bone marrow smears (left panels) and hematoxylin and eosin (H&E) stains of bone marrow sections (right panels) of indicated mice (>1.5 years). Arrows indicate dysplastic granulocytes. Scale bars represent 30 μm. The stains were representatives of 3 animals from each genotype.