Outcome and immune reconstitution of patients enrolled in the study. (A) Cumulative incidence (CI) of aGVHD in the study population. (B) Probability of disease-free survival in the study population. (C) Reconstitution kinetics of αβ⁺ and γδ⁺ T cells, as well as NK cells and B lymphocytes, in children receiving T-cell receptor (TCR)–αβ/CD19-depleted haploidentical HSCT. Peripheral blood samples were obtained 1, 3, 6, 9, and 12 months after HSCT. NK cells were identified and counted on the basis of their CD3^–CD56⁺ phenotype. The graph depicts the absolute number of each cell subset, which is shown in terms of mean and standard error of the mean (SEM). Absolute numbers were calculated by multiplying the frequency of each individual cell subset by the white blood cell count. (D) Reconstitution kinetics of naïve and memory CD4⁺ and CD8⁺ T cells in children receiving TCR-αβ/CD19–depleted haploidentical HSCT. Peripheral blood samples were obtained 1, 3, 6, 9, and 12 months after HSCT. Naïve and memory T cells were identified and counted on the basis of their reciprocal expression of the two CD45 isoforms (CD45RA and CD45RO, respectively). The graph depicts the absolute number of each cell subset, which is shown in terms of mean and SEM. Absolute numbers were calculated by multiplying the frequency of each individual cell subset by the white blood cell count. DFS, disease-free survival; E, events; Pts., number of patients evaluable for the occurrence of immune reconstitution.