Ezh2 differentially controls cell survival of effector and regulatory T cells. (A) Live cell and cytokine staining in Th0 cells at day 5 in the presence of apoptosis or necrosis inhibitors (left panel). Percentages of live cells in 3 independent experiments (unpaired Student t test: **P < .01, ***P < .001) (right panel). (B) Western blot of death receptor–related cell death proteins in Th0 and iTreg cells from Ezh2^fl/fl and Ezh2^fl/fl.CD4Cre cells. (C) Live cell and cytokine staining in iTreg cells at days 4 to 5. (D) Western blot of intrinsic cell death proteins in naïve Th0 and iTreg cells from Ezh2^fl/fl and Ezh2^fl/fl.CD4Cre cells. (E) Log2 ratio of live Ezh2^fl/fl.CD4Cre and WT cells at days 5 and 6 in mixed-cell cultures under Th0 conditions. (F) Model summary of regulation of IFN-γ production and cell death by Ezh2 in effector Th cells.