Association of genes regulated by Ezh2 and trimethylation of histone 3 lysine 27. (A) Gene expression in Ezh2^fl/fl.CD4Cre Th0 vs iTreg cells, relative to Ezh2^fl/fl cells. Each point represents a single gene. Red dots denote genes significantly differentially expressed (FDR adjusted P < .05) in both Th0 and iTreg cells; green dots denote genes only differentially expressed in iTreg cells; purple dots denote genes only differentially expressed in Th0 cells. Selected genes belonging to relevant gene sets are named. (B) Change in expression in Ezh2^fl/fl.CD4Cre Th0 cells relative to Ezh2^fl/fl vs change in H3K27me3 of Ezh2^fl/fl Th0 cells relative to Ezh2^fl/fl.CD4Cre. Each point represents a single gene. Red dots denote genes significantly less methylated in Ezh2^fl/fl.CD4Cre and upregulated; green dots denote genes significantly less methylated in Ezh2^fl/fl.CD4Cre but not differentially expressed; purple dots denote genes significantly differentially expressed with no change in H3Kme3; gray dots denote genes that do not show a significant change in either expression or H3K27me3. (C-D) ChIP-qPCR showing H3K27me3 and H3K4me3 marks at Th1, Th2, and control loci. Data shown are mean ± SEM of 3 independent experiments (paired Student t test: *P < .05, **P < . 01).