Plg promotes phagocytosis of IgG-coated microbeads by J774A.1 cells. J774A.1 macrophage-like cells were pretreated with or without Plg (1 μM) for 24 hours in the presence or absence of TXA (200 μM) or PI (20 nM). (A) Confocal microscopic images of macrophages with ingested IgG-opsonized green latex beads. Images at original magnification of ×63 were captured as in Figure 1. The plasma membrane is stained with anti-CD45 (red). The cells are counterstained with 4′,6 diamidino-2-phenylindole (blue). (B) Quantification (means ± SD) of IOD of ingested beads per macrophage. Results are representative of 3 independent experiments.