NOX-A12 induces release of CXCL12 from murine stromal cell lines. (A-C) The murine BMSCs MS-5, 9-15C, and TSt-4 were incubated with 100 nM NOX-A12 for the indicated time spans leading to the release of CXCL12. The dashed lines indicate the basal CXCL12 secretion levels. (D) CXCL12 was rapidly released within 5 minutes after incubation with 100 nM NOX-A12. (E) MS-5 cells were incubated with various concentrations of NOX-A12 for 30 minutes. Results indicate the concentration of CXCL12 in supernatants quantified by ELISA. Data are representative of 3 independent experiments.