NOX-A12 inhibits CXCL12-dependent chemotaxis of primary CLL cells and cell lines. (A) NOX-A12 significantly inhibited chemotaxis of primary CLL cells toward CXCL12. CLL cells from 12 different patients were allowed to migrate toward 25 nM CXCL12 that was preincubated with different concentrations of NOX-A12. The box plots represent the median including interquartile range, maximum, and minimum of migrated CLL cells. The Wilcoxon matched-pairs signed-ranks test was used for statistical description. (B) The acute T-cell leukemic cell line Jurkat, and (C) the pre-B ALL cell line Nalm-6 were assayed for chemotaxis toward CXCL12. Results indicate relative migration compared with control samples migrating to 0.3 nM CXCL12 and samples preincubated with different concentrations of NOX-A12, representing the mean ± SD values (n = 3). Data are representative of 3 or more independent experiments.