Conditional deletion of pfn1 in HSCs. (A) Schematic of pfn1 floxed allele showing deletion of floxed exon 1 following Cre recombinase activity. Use of Scl-Cre-ER^T results in specific deletion of the pfn1 gene in HSCs following tamoxifen treatment. (B) Genotype analyses of LT-HSCs (Lin⁻Sca-1⁺Kit⁺Flk2⁻CD34⁻, LSKFC) and Lin⁺, Lin⁻, Lin⁻Kit⁺ (LK) cells and whole BM (WBM) cells at 1 and 6 days after tamoxifen injections. Deletion of pfn1 is indicated by the arrow (700 bp).