Regulation of C/EBP-ε by miR-130a in human granulocytic precursors. (A-B) Transient transfection of MB/PM cells with LNA-miR-130a/LNA-miR-neg. The cells were harvested 4 hours after transfection. (A) Western blot analysis shows C/EBP-ε and β-actin expression in LNA-miR-neg and LNA-miR-130a transfected MB/PM cells. (B) Real-time-PCR measurements of LTF, CAMP, and CYBB. Error bars indicate SD. (C-D) MC/MM cells transiently transfected with pre-miR-130a/pre-miR-neg. The cells were harvested after 24 hours. (C) Western blot analysis examination on C/EBP-ε and β-actin. (D) Real-time-PCR measurements on LTF and CAMP. Error bars indicate SD. (E-F) MC/MM cells transiently transfected with LNA-miR-130a/LNA-miR-neg. Cell harvest after 24 hours after transfection. (E) Western blot analysis examination on C/EBP-ε and β-actin. (F) Real-time-PCR measurements on LTF and CAMP. Error bars indicate SD. The expressions from the transfections with the negative controls (pre-miR-neg and LNA-miR-neg) were assigned the value 1 and the relative expression measured from the transfected with pre-miR-130a or LNA-miR-130a was recalculated accordingly. Asterisks indicate significant differences in expression between transfections (*P < .05).