BAF complexes expressed in quiescent LT-HSCs are assembled around the BRAHMA (BRM) ATPase. (A) Developmental hierarchy of the (B-C) BM populations analyzed. (B) BAF gene expression in purified populations of mouse BM cells, as determined by quantitative reverse-transcription polymerase chain reaction. Relative quantity (RQ) values represent the relative expression of each gene in the different populations analyzed over total BM cells after GAPDH normalization. BAF genes were ordered according to their expression level in quiescent LT-HSCs. Oligo specificity was tested on complementary DNAs encoding alternative family members. Gray boxes: undetectable expression. Brain: mouse embryonic brain (E14.5). Purity of all sorted population was evaluated to 98% to 99% upon reanalysis. Frequency of all sorted long-term repopulating hemopoietic stem cells (LT-HSC) populations was 1 in 4. (C) ΔCT values for each gene were determined relative to GAPDH in each population (done in triplicate). (D) Schematic representation of BAF complexes expressed in quiescent LT-HSCs (hscBAF) assembled around the BRM ATPase. See also supplemental Tables 5-7. BM, bone marrow; δCT, delta cycle threshold; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.