Long-term engraftment kinetics of germline JAK2V617I CD34+ cells after xenograft transplantation. Human CD45 engraftment kinetics for age-matched NBM cases: (A) controls, n = 2 subjects; 2 recipient mice per sample and (B) JAK2V617I, n = 4, cases C1-C4; 1-3 recipient mice per samples after injection of 100 000 CD34+ cells into NOD/LtSz-scidIL2Rgnull (NSG) mice. P values in (B) represent a comparison of JAK2V617I and NBM engraftment levels at each respective time. (C) Engraftment kinetics of CD19+ B cell, CD15/33/66b+ myeloid cells, and CD34+ progenitor cells expressed as a percentage of total hCD45+ cells. (D) Quantification of HSCs, MEPs, GMPs, and CMPs in the BM at 18 weeks after transplantation. Error bars represent SEM. P values are indicated if < .1.