![Figure 6. HSCs with loss of both Apc and β-catenin show an enhanced repopulation capacity. (A) Diagram for the experimental design. CM were generated by transplanting an equal number of wild-type CD45.1+CD45.2+ BM cells (from primary mice) and CD45.2+ BM cells from Mx1-Cre Apcfl/flβ-cateninfl/fl, Mx1-Cre β-cateninfl/fl, or β-cateninfl/fl transplanted mice 2 months after induction. These transplanted mice have undergone 1 round of transplantation. (B) Flow cytometric analysis of CD45.2+CD45.1− (donor cells) and CD45.1+CD45.2+ (competitor cells) PB cells from representative CM 1 month after transplantation. The numbers indicate the percentage of cells in each population. (C) Histogram shows the relative ratio of CD45.2+CD45.1− vs CD45.1+CD45.2+ PB cells in CM examined at 1 to 3 months after the first transplantation (left) and at 1, 2, and 4 months after secondary transplantation (right; mean ± standard deviation [SD], n = 4-5). (D) The ratio of CD45.2+CD45.1− vs CD45.1+CD45.2+ in myeloid cells, B cells, and T cells in PB from first and second recipient mice were analyzed (mean ± SD, n = 4-5). The significant differences between the cells from ApcΔ/Δβ-cateninΔ/Δ and β-cateninΔ/Δ are indicated. (E) Lineage differentiation in the recipient mice transplanted with BM cells from Mx1-Cre Apcfl/flβ-cateninfl/fl, Mx1-Cre β-cateninfl/fl, or β-cateninfl/fl CM. Histogram shows percentage of donor-derived (CD45.2+CD45.1−) myeloid cells (Gr1+Mac+), B cells (B220+), and T cells (CD3+) in PB analyzed 2 and 4 months after the secondary transplantation (mean ± SD, n = 3-5). (F) Flow cytometric analysis of CD45.2+CD45.1− and CD45.1+CD45.2+ cells in LSK (lin−Sca-1+c-Kit+) and HSC (LSKCD34−) stem cell–enriched population in representative recipient mice. (G) The ratio of CD45.2+CD45.1− and CD45.1+CD45.2+ cells in total BM cells, Lin− cells, HPCs (lin-Sca-1−c-Kit+ cells), LSKs, and HSCs from the recipients 5 months after the first transplantation is shown (mean ± SD, n = 3). The significant differences between ApcΔ/Δβ-cateninΔ/Δ and β-cateninΔ/Δ or β-cateninfl/fl are indicated. *P < .05; **P < .01; ***P < .001.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/20/10.1182_blood-2012-12-473470/2/4063f6.jpeg?Expires=1724238393&Signature=4rpl73WQMyl3r4vsaloVInXMMmYf5oA8lhlqXj-hPg5hT6TVgewUv~~8PFR2dhGC-LdCPVDE5pcg0KgOouoZmCk5zehZCKq0QHVRpL2TTr9ZDLJPnBkbEIkqKk0VAK1SitvOWfPDfODYjgjBdiuCBndOKfQQTlinihzdEzHL1hPNVPgrqM~9TjH~t3GyP2DrLRq6S~4Mbr6DaLZb2h8z3qaEghYTIh1utNtZB4mIm08MWYIlN8SbmnysQgAMc4cDKe92mEbecW-h49laSPQkVDuzqbC-4E1oKooMZGxh82KaQ79KuMNb2anbV-T-ADRvYv4j39inF4JmELz7XJQtWg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HSCs with loss of both Apc and β-catenin show an enhanced repopulation capacity. (A) Diagram for the experimental design. CM were generated by transplanting an equal number of wild-type CD45.1+CD45.2+ BM cells (from primary mice) and CD45.2+ BM cells from Mx1-Cre Apcfl/flβ-cateninfl/fl, Mx1-Cre β-cateninfl/fl, or β-cateninfl/fl transplanted mice 2 months after induction. These transplanted mice have undergone 1 round of transplantation. (B) Flow cytometric analysis of CD45.2+CD45.1− (donor cells) and CD45.1+CD45.2+ (competitor cells) PB cells from representative CM 1 month after transplantation. The numbers indicate the percentage of cells in each population. (C) Histogram shows the relative ratio of CD45.2+CD45.1− vs CD45.1+CD45.2+ PB cells in CM examined at 1 to 3 months after the first transplantation (left) and at 1, 2, and 4 months after secondary transplantation (right; mean ± standard deviation [SD], n = 4-5). (D) The ratio of CD45.2+CD45.1− vs CD45.1+CD45.2+ in myeloid cells, B cells, and T cells in PB from first and second recipient mice were analyzed (mean ± SD, n = 4-5). The significant differences between the cells from ApcΔ/Δβ-cateninΔ/Δ and β-cateninΔ/Δ are indicated. (E) Lineage differentiation in the recipient mice transplanted with BM cells from Mx1-Cre Apcfl/flβ-cateninfl/fl, Mx1-Cre β-cateninfl/fl, or β-cateninfl/fl CM. Histogram shows percentage of donor-derived (CD45.2+CD45.1−) myeloid cells (Gr1+Mac+), B cells (B220+), and T cells (CD3+) in PB analyzed 2 and 4 months after the secondary transplantation (mean ± SD, n = 3-5). (F) Flow cytometric analysis of CD45.2+CD45.1− and CD45.1+CD45.2+ cells in LSK (lin−Sca-1+c-Kit+) and HSC (LSKCD34−) stem cell–enriched population in representative recipient mice. (G) The ratio of CD45.2+CD45.1− and CD45.1+CD45.2+ cells in total BM cells, Lin− cells, HPCs (lin-Sca-1−c-Kit+ cells), LSKs, and HSCs from the recipients 5 months after the first transplantation is shown (mean ± SD, n = 3). The significant differences between ApcΔ/Δβ-cateninΔ/Δ and β-cateninΔ/Δ or β-cateninfl/fl are indicated. *P < .05; **P < .01; ***P < .001.
