Figure 4
![Figure 4. Loss of β-catenin prevents rapid exhaustion of Apc-deficient HSCs in vivo. (A) Flow cytometric analysis of the frequency of stem cell-enriched population (Lin-c-Kit+Sca+ [LSK]) and HSCs (LSK CD150+CD48−) in BM from representative ApcΔ/Δ and ApcΔ/Δβ-cateninΔ/Δ mice 10-14 days after induction. (B-D) The histograms depict the total number of HSCs in BM (B) from ApcΔ/Δ and ApcΔ/Δβ-cateninΔ/Δ mice 10-14 days after induction (mean ± standard deviation [SD], n = 5-8), (C) from ApcΔ/Δβ-cateninΔ/Δ, β-cateninΔ/Δ, and β-cateninfl/fl mice 5 weeks after induction (mean ± SD, n = 3), and (D) from ApcΔ/Δβ-cateninΔ/Δ (6 months after induction) and Mx1-Cre Apcfl/flβ-cateninfl/fl (mean ± SD, n = 2-4). *P < .05; ***P < .001.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/20/10.1182_blood-2012-12-473470/2/4063f4.jpeg?Expires=1726828257&Signature=p7H9-6cMicbZ5ilDWe0dCGvwy2xL~UuMj4GODK7sdlQ~4P9dqhtyCQTmWV5bGWGI75sjcE4Ku7FuNL08PsTjwzQXaDYpGfSCVZ1JImgDyhs~adZFH-P5OjrtAAkBWmCUOnV3SA8hp0LuOgSm~-XIZlMVgTTLnHqWfKiAMes3sJvy8BYP~6rHhJBtcXlw1pHe7kOmwOVZC0SJcDGed3A1fQkMRub1Ay~UYWPg9oH~Wp~r0JKuZ4ThFpSR0GkFooUYsQsMcyxHHd8fPYypypD6L2nMOpd9xI5rqvS9A3UGpzd8R1qgsCx8zSufskbm3BAKf-OxYPpI2tZqGdJzDZEclA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Loss of β-catenin prevents rapid exhaustion of Apc-deficient HSCs in vivo. (A) Flow cytometric analysis of the frequency of stem cell-enriched population (Lin-c-Kit+Sca+ [LSK]) and HSCs (LSK CD150+CD48−) in BM from representative ApcΔ/Δ and ApcΔ/Δβ-cateninΔ/Δ mice 10-14 days after induction. (B-D) The histograms depict the total number of HSCs in BM (B) from ApcΔ/Δand ApcΔ/Δβ-cateninΔ/Δ mice 10-14 days after induction (mean ± standard deviation [SD], n = 5-8), (C) from ApcΔ/Δβ-cateninΔ/Δ, β-cateninΔ/Δ, and β-cateninfl/fl mice 5 weeks after induction (mean ± SD, n = 3), and (D) from ApcΔ/Δβ-cateninΔ/Δ (6 months after induction) and Mx1-Cre Apcfl/flβ-cateninfl/fl (mean ± SD, n = 2-4). *P < .05; ***P < .001.
