Loss of β-catenin rescues Apc-deficient mice. (A) Comparison of engraftment efficiency of CD45.2 Mx1-Cre Apc^fl/fl, Mx1-Cre Apc^fl/flβ-catenin^fl/fl, Mx1-Cre β-catenin^fl/fl, or β-catenin^fl/fl BM cells in wild-type recipient mice (CD45.1) by flow cytometric analysis of peripheral blood (PB) at 6 weeks after transplantation (mean ± standard deviation, n = 4-6). (B) Analysis of deletion of Apc or β-catenin as determined by PCR analysis of genomic DNA from BM LSKs 7 days after the last injection of pI-pC. (C) Kaplan-Meier survival curve of recipient mice reconstituted with BM cells from Mx1-Cre Apc^fl/fl, Mx1-Cre Apc^fl/flβ-catenin^fl/fl (n = 13), Mx1-Cre β-catenin^fl/fl, or β-catenin^fl/f (n = 5-10) mice after 3 doses of pI-pC injection. Arrowheads indicate pI-pC injections.