siRNA-mediated knockdown of IRF7, MAP2K6, and MAP2K3 reduces the expression of ISGs induced by Tat in primary iDC and primary MDM. (A) mRNA levels of different genes in iDC infected with Ad-tTA or Ad-TatSF2+Ad-tTA and treated with siRNA, reported as fold induction relative to those observed in Ad-tTA infected iDC, treated with nonspecific siRNA. The average of three independent experiments is reported. (B) Flow cytometric protein expression analysis in iDC infected with Ad-tTA or Ad-TatSF2+Ad-tTA and treated with siRNA, reported as median fluorescence intensity (MFI). (C) mRNA levels of different genes in MDM infected with Ad-tTA or Ad-TatSF2+Ad-tTA and treated with siRNA, reported as fold induction relative to those observed in Ad-tTA infected MDM, treated with nonspecific siRNA. The average of three independent experiments is reported. (D,E) Flow cytometric protein expression analysis in MDM infected with Ad-tTA or Ad-TatSF2+Ad-tTA and treated with siRNA, reported as MFI.