Imprinted allogeneic T cells show differential in vivo homing. (A) Donor T-cell numbers in the liver at day 7 after BMT are highest in the liver DC group (*P < .001, n = 4-5). (B) Apoptosis as analyzed by staining for annexin V is comparable among the groups. (C) On day 7, immunohistochemical staining and quantification of the number of CD3⁺ cells per high-power field shows significantly higher numbers of allogeneic T cells in the small and large bowel of mice in the MLN DC group compared with the PLN DC group (*small bowel: P < .001, large bowel: P < .001, n = 5). Corresponding representative photomicrographs (original magnification: × 200). Images were visualized with an Olympus BX40 microscope (Olympus, Melville, NY) equipped with a 10×/0.65 aperture objective lens. Images were acquired with a JVC digital camera GC-Qx 5HDU (JVC, Wayne, NJ). (D) The expression of the homing molecules LPAM-1 and P-lig on donor T cells in vivo was analyzed at day 6 after pre-vious stimulation with different organ-derived dendritic cells in vitro (n = 5). Both molecules are up-regulated irrespective of previous stimulation in vitro. Error bars represent SEM.