BAFF and chemokines differently activate NF-κB1 and NF-κB2 pathways. Mock-treated B cells were stimulated for 2 minutes with medium, 100 ng/mL CCL21, 100 ng/mL CXCL13, or 50 ng/mL BAFF. The p52 contents of total cell lysates were corrected for the actin contents. Phosphorylation of AKT, IκBα, p38MAPK, ERK1/2, PKCθ, and PLCβ3 were corrected for total relevant protein. Blots from one representative experiment are shown. Values reported for p52 contents or phosphorylation are mean values plus or minus SD from 2 (PLCβ3) or 3 independent experiments. Values reported for PLCγ2 phosphorylation were calculated as the ratio between PLCγ2 contents in 4G10 immunoprecipitates and those in total lysates. Results are mean values plus or minus SD from 2 independent experiments. Black dividing lines between lanes represent the cuts resulting from the grouping of images from different parts of the same gel in order to optimize the order of the bands and/or remove lanes corresponding to conditions not relevant to the present work.