VX-680 induces cellular caspase activation and increases Bax/Bcl-2 ratio in AML cells. (A)VX-680 inhibits cell proliferation in a dose-dependent manner. HL-60 cells were incubated with increasing doses of VX-680 or DMSO for 72 hours. Cell survival rate was measured by MTT assay. (B) VX-680 induces apoptosis in HL-60 cells. Cells were incubated with VX-680 and collected at time points as indicated. The apoptosis was assessed as sub-G1 population by flow cytometry. VX-680 induces HL-60 cells apoptosis in both dose-dependent and time-dependent manners. (C-D) AML HL-60 and U937 cells were treated with increasing concentrations of VX-680. Cells were collected, lysed, and subjected to Western blot analysis with cleaved-caspase3, cleaved-PARP, p-Akt-1(Ser473), Bax, Bcl-2 specific antibodies. GAPDH was used as a loading control. Data shown are representative of 3 independent experiments.
