Transduction of ZAP-70⁻ CLL cells with Ad-ZAP-70 versus Ad-ZAP-70-YF²⁹² and measurement of anti-μ–induced increases in phosphorylated p72Syk, BLNK, or PLC-γ. (A) Immunoblot ana-lysis for ZAP-70 (top row) in lysates of CLL cells before or after transduction with Ad-ZAP-70 or Ad-ZAP-70-YF,²⁹² as indicated at the top of each lane. The blots were stripped and then reprobed with anti–β-actin to monitor for protein loading (bottom row), as indicated on the left of each blot. (B-D) Anti-μ–induced percentage increases in phosphorylated p72^Syk (B), BLNK (C), or PLC-γ (D) detected in ZAP-70⁻ CLL B cells that were transduced with the control vector or Ad-ZAP-70-YF²⁹² (left panel) or with Ad-ZAP-70 or Ad-ZAP-70-YF²⁹² (right panel). Each symbol represents the percentage increase in phosphorylated protein detected in an individual CLL cell sample. The lines connect the percentage increase in detected phosphorylated protein of anti-μ–treated CLL cells of the same sample transduced with each of the different vectors. The differences in the mean percentage increase of phosphorylated protein upon anti-μ treatment in control vector–transduced CLL cells versus that of Ad-ZAP-70-YF²⁹²–transduced CLL cells was significant (P < .05). However, there was not a significant difference in the mean percentage increase of phosphorylated protein upon anti-μ treatment of Ad-ZAP-70–transduced CLL cells versus that of Ad-ZAP-70-YF²⁹²–transduced CLL cells.