![IgM-induced calcium mobilization in ZAP-70− CLL B cells transduced with Ad-ZAP-70 or Ad-ZAP-70-KA369. (A) The fluorescence of labeled CLL cells induced by treatment with anti-μ is indicated on the y-axis over time (in seconds, as indicated on the x-axis). The horizontal dotted line in each panel provides the basal fluorescence of labeled cells prior to treatment. The arrow indicates the time when the cells were treated with anti-μ (IgM). The top panel shows the changes in calcium flux ([Ca2+]i) detected in ZAP-70− CLL cells transduced with Ad-lacZ (Control); the middle panel shows the [Ca2+]i observed in the same CLL sample transduced with Ad-ZAP-70; and the bottom panel shows the [Ca2+]i observed in the same CLL sample transduced with Ad-ZAP-70-KA369. (B) Peak increases in fluorescence intensity over the basal level in ZAP-70− CLL cells transduced with Ad-lacZ (Control) versus that of CLL cells transduced with Ad-ZAP-70 (top) or Ad-ZAP-70-KA369 (bottom). The lines connect the data points obtained from the same CLL sample, but transduced with Ad-lacZ (control) or Ad-ZAP-70 or Ad-ZAP-70-KA369, as indicated at the bottom of the panels.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/5/10.1182_blood-2006-12-062265/2/zh80040814770004.jpeg?Expires=1720367268&Signature=kfDt8sHDzd-6a-QDwavKP3qHQ3gJsW1CEm6Yvb8o8Bd-uhY9lwtmyS6xA8PCiMMnH98myk2Sw66z2kSmih8DIV2PVvmcAq-8FEUMIEYytJgMW7JTeiyIAo0qDHom-~Q8Q-FWnJFyQd8Fr78ro~fRqyR2W4RF9EZ~D7l8CCW7RNjc-grh~RmcpLPDOFWU6WUqLq3R6VCeyQcy3kNR1nakmuNR6pfadOvzsoHeulerDTULSxn5Zv~~O~H47QHBsD1kRMOey9zSdYjM9xzJW9QjvoaTsm9yQQlAHixNcu~LaCHvaf5kgbKZWBCid4zhbiAp5sHp3rXalvjeLTRs-qQB1w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IgM-induced calcium mobilization in ZAP-70− CLL B cells transduced with Ad-ZAP-70 or Ad-ZAP-70-KA369. (A) The fluorescence of labeled CLL cells induced by treatment with anti-μ is indicated on the y-axis over time (in seconds, as indicated on the x-axis). The horizontal dotted line in each panel provides the basal fluorescence of labeled cells prior to treatment. The arrow indicates the time when the cells were treated with anti-μ (IgM). The top panel shows the changes in calcium flux ([Ca2+]i) detected in ZAP-70− CLL cells transduced with Ad-lacZ (Control); the middle panel shows the [Ca2+]i observed in the same CLL sample transduced with Ad-ZAP-70; and the bottom panel shows the [Ca2+]i observed in the same CLL sample transduced with Ad-ZAP-70-KA369. (B) Peak increases in fluorescence intensity over the basal level in ZAP-70− CLL cells transduced with Ad-lacZ (Control) versus that of CLL cells transduced with Ad-ZAP-70 (top) or Ad-ZAP-70-KA369 (bottom). The lines connect the data points obtained from the same CLL sample, but transduced with Ad-lacZ (control) or Ad-ZAP-70 or Ad-ZAP-70-KA369, as indicated at the bottom of the panels.
