Detection of the e6a2 transcript. M is a 100-bp ladder marker. A no RNA negative control (nr) and a no template (nt) negative control are shown. Amplification of the usual e1a2 transcript (PCR product of 389 bp) from the ALL-1 cell line, and absence of product from the HL-60 cell line are shown in lanes 7 and 8, respectively. The abnormal 1033-bp band seen at presentation in blood and bone marrow is demonstrated in lanes 1 and 2. Lane 3 reveals the absence of abnormal band from the remission bone marrow after initial therapy. A positive control sample is shown in lane 4.