Western blotting of DLBCL biopsy samples using the JC12 monoclonal antibody. The JC12 immunohistochemistry (IHC) scoring for FOXP1 expression in routinely fixed tissue from each case was scored as + for strongly positive, +/− for weak/moderate, c for cytoplasmic, or − for negative, and the subtyping as non-GC (N) or GCB (G) was performed as described previously.⁹  The arrowhead indicates the position of the full-length FOXP1 protein, while the arrow indicates the smaller isoforms. The blots were reprobed with antiactin as a loading control.