Cytotoxic cells could be generated from the EBV-infected LTB₄-containing cultures. EBV-infected CBMCs were cultured with LTB₄ (100 nM) and restimulated twice (on seventh and 14th day of culture) with irradiated autologous EBV-infected B cells at a ratio of 10:1. Starting on the ninth day of culture, IL-2 (20 U/mL) was added every third day. The 20-day-old cultures were depleted of B cells. The cytotoxic effect of the residual cells was tested against autologous EBV-infected B cells (○), preincubated with mAb W6/32 () and preincubated with the mAb CR3/43 (●); and with autologous B cells activated with CD40L and IL4 (□), K562 cells (▲), and allogeneic LCLs CBM1 (×) and LCL2996 (◇).