The involvement of LTB4 in T- and NK-cell activation in the EBV-infected PSK- and Trx80-containing cultures. (A) SAP expression. Immunoblot of lysates corresponding to 1.5 × 105 cells, harvested on the 6th day of culture. EBV-infected CBMCs were cultured with PSK (25 μg/mL) or Trx80 (100 nM). MK886 (1 μM), BWA4C (100 nM), and LTB4 (100 nM) were added as indicated. Jurkat cells and LCLs were used as positive and negative controls, respectively. The results show 1 representative of 3 experiments. (B) IL-15 and IL-12 in the supernatant of 3-day-old cultures were tested by ELISA. The results represent the means plus or minus SD of 3 independent experiments. *P < .05 and **P < .01 compared with PSK- or Trx80-treated cultures. (C) Inhibition of B-cell proliferation induced by EBV. 3H-thymidine incorporation was tested on the 6th day of culture. 3H-thymidine was present during the final 14 hours. The cultures were initiated with 2 × 105 cells. Means plus or minus SD of 3 independent experiments are shown. * indicates Pless than .05 compared with untreated cultures.
