The genetic suppressor element GSE24-2 increases survival against cisplatin and alters the kinetics of JNK and p38 activation. (A) Diagram of the dyskerin cDNA that shows the localization of GSE24-2 and the construct DKC 5′. (B) pLNCX, DKC, DKC 5′, and GSE24-2 cell lines were treated with increasing amounts of cisplatin. Survival was measured 72 hours after treatment. Data represent the means and standard deviations of 2 experiments performed in quadruplicate. (C) Cells were cultured in serum-free medium for 16 hours and then treated with several doses of cisplatin for 6 hours. p-JNK, p-p38, and JNK1 were detected by immunoblot using specific antibodies. The experiments were repeated 3 times, with similar results. (D) Comparison of amino-acid sequences from the pseudouridine synthase domain of GSE24-2 and the corresponding regions from other organisms.