Role of MyD88 in regulating IL-10 and IL-6 production by E coli–treated and adenosine-stimulated macrophages. (A) MyD88 is not necessary for the synergistic effect of adenosine and E coli on IL-10 production. Peritoneal macrophages were taken from MyD88 KO and WT mice. After 5 hours of stimulation with heat-killed E coli or E coli plus adenosine IL-10 enzyme-linked immunosorbent assay was performed. ***P < .001 vs. corresponding adenosine-untreated, E coli–stimulated groups. (B) MyD88 is partially required for E coli–induced IL-6 production. IL-6 cytokine levels were determined from the same supernatants that were used for IL-10 detection. ^###P < .001 vs. E coli–treated MyD88 WT group, ^##P < .01 vs. adenosine/E coli–treated MyD88 WT group. Results (mean ± SEM) shown are representative of at least 3 experiments with n = 6 in each experiment.