PSF content in cytoplasmic and nuclear extracts derived from human cell lines. Nuclear and cytoplasmic extracts were prepared from the NPM/ALK⁻ cell line, Jurkat, and the NPM/ALK⁺ cell lines, SUDHL-1 and JB6. (A) Total cell lysates (lanes 1-3), cytoplasmic extracts (lanes 4-6), and nuclear extracts (lanes 7-9) were resolved by SDS-PAGE, and PSF was detected by Western blotting (top panel). The purity of the extracts were controlled by Western blotting with anti-β-actin for the cytoplasm (middle panel) and anti-histone H1 for the nucleus (bottom panel). (B) Nuclear and cytoplasmic extracts from SUDHL-1 cells were probed with antiphosphotyrosine antibody.